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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GTF2I / TFII I Antibody (aa985‑998) LS‑C288145
TFII I antibody LS-C288145 is an unconjugated rabbit polyclonal antibody to TFII I (GTF2I) from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C288145-100
100 µl (0.2 mg/ml)
Unavailable

Popular GTF2I / TFII I Products

Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Species: Human, Mouse, Dog, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Anti-GTF2I / TFII I antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Western blot analysis of extracts of 293 cells tissue.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA

Product Description

TFII I antibody LS-C288145 is an unconjugated rabbit polyclonal antibody to TFII I (GTF2I) from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
About GTF2I / TFII I
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). P78347 NM_032999 NP_127492.1

GTF2I Antibody, BAP-135 Antibody, BAP135 Antibody, BTK-associated protein, 135kD Antibody, BTK-associated protein 135 Antibody, DIWS Antibody, IB291 Antibody, SRF-Phox1-interacting protein Antibody, WBSCR6 Antibody, TFII I Antibody, TFII-I Antibody, BTKAP1 Antibody, WBS Antibody, GTFII-I Antibody, SPIN Antibody


Specifications

Target
Human GTF2I / TFII I
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 5 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa985-998
Specificity
Region between residue 985 and 998 of human general transcription factor II-I using the numbering given in entry NP_127492.1 (GeneID 2969).
Usage
Immunohistochemistry: Antigen retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Human controls: Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Colon Carcinoma, Ewing.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-buffered saline, 0.1% BSA, 0.09% sodium azide.
Storage
Store at 2-8°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.

Immunohistochemistry

Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.

Immunohistochemistry

Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.

Immunohistochemistry

Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human Ewing sarcoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.


Requested From: 
Date Requested: 6/18/2018

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