Human GSK3A / GSK3 Alpha

Rabbit

Human (tested or 100% immunogen sequence identity)

Chimpanzee, Rat (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.

pSer21

This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100% homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined.

• Immunofluorescence
• Western blot (1:500 - 1:3000)
• ELISA (1:2000 - 1:10000)

This affinity-purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kD in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS21 of GSK3A.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P49840 NCBI: NM_019884 NP_063937.2