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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C18920-100 100 µg (1.28 mg/ml) $425 
GSK3A / GSK3 Alpha Antibody - Anti-GSK3A Antibody - Western Blot. Western blot of Affinity Purified anti-GSK3A antibody shows detection of a 52 kD band corresponding to human GSK3A in various human derived 293T cell extracts. Cells were serum starved for 24 h. Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min. Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when an inhibitor is added (lane 3). Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-GSK3A antibody. Signal was detected using standard techniques. Personal communication, Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada.

Polyclonal GSK3A / GSK3 Alpha Antibody (phospho‑Ser21, IF, WB) LS‑C18920

Polyclonal GSK3A / GSK3 Alpha Antibody (phospho‑Ser21, IF, WB) LS‑C18920

Note: This antibody replaces LS-C66826, LS-C66825
Antibody:
Rabbit Polyclonal to Human GSK3A / GSK3 Alpha
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18920-100
100 µg
1.28 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GSK3A / GSK3 Alpha
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GSK3 Alpha antibody LS-C18920 is an unconjugated rabbit polyclonal antibody to human GSK3 Alpha (GSK3A ). Validated for ELISA, IF and WB.
Target
Human GSK3A / GSK3 Alpha
Synonyms
GSK3A | GSK-3 alpha | GSK3 alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • ELISA (1:2000 - 1:10000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.
Epitope
pSer21
Specificity
This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100% homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined.
Usage
This affinity-purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kD in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS21 of GSK3A.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GSK3A / GSK3 Alpha
P49840 NM_019884 NP_063937.2

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Requested From: United States
Date Requested: 2/22/2020