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Catalog Number Size Price
LS-C23951-100 100 µg $739 
Antibody

Monoclonal Mouse anti‑Drosophila GSK3A / GSK3 Alpha Antibody (aa203‑219, WB) LS‑C23951

Monoclonal Mouse anti‑Drosophila GSK3A / GSK3 Alpha Antibody (aa203‑219, WB) LS‑C23951

Antibody:
GSK3A / GSK3 Alpha Mouse anti-Drosophila Monoclonal (aa203-219) Antibody
Application:
ICC, WB
Reactivity:
Drosophila, Insect
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$739
LS-C23951-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
GSK3A / GSK3 Alpha Mouse anti-Drosophila Monoclonal (aa203-219) Antibody
Application:
ICC, WB
Reactivity:
Drosophila, Insect
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GSK3 Alpha antibody LS-C23951 is an unconjugated mouse monoclonal antibody to GSK3 Alpha (GSK3A) (aa203-219) from drosophila. It is reactive with drosophila and insect. Validated for ICC and WB.
Target
Drosophila GSK3A / GSK3 Alpha
Synonyms
GSK3A | GSK-3 alpha | GSK3 alpha
Host
Mouse
Reactivity
Drosophila, Insect (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa203-219 (18aa, C-KQLLHGEPNVSYICSRY) derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK-3/ shaggy enzyme (ovalbumin coupled). Percent identity by BLAST analysis: Drosophila, Mosquito (100%); Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).
Epitope
aa203-219
Specificity
Recognizes Glycogen Synthase Kinase 3 (GSK3). Due to extremely high conservation among the known enzymes of many different species, should cross-react with nearly all species homologues. Species cross-reactivity: Mouse, rat, S. pombe, Dictyostelium and human.
Applications
  • ICC (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
Failed Applications
  • Immunoprecipitation
Usage
WB: Detects GSK-3 in RIPA lysates from human Jurkat and A431, rat L6 and PC12 and mouse 3T3 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with Mouse Anti-Glycogen Synthase Kinase 3 (0.5 ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. ICC: Demonstrated positive immunostaining for GSK3 in L6 cells fixed with acetone.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GSK3A / GSK3 Alpha
P49840 NM_019884 NP_063937.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/22/2021