Human GRIA4 / GLUR4

Goat

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit, Chicken (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3NP_001070711.2NP_001070712.1.

aa279-292

Human GRIA4 / GLUR4. This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 2 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Immunohistochemistry: LS-B3606 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3606 was determined to be 5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 90 kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kD according to NP_001070711.1). Recommended concentration: 0.3-1 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P48058 NCBI: NM_000829 NP_000820.3