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Antibodies > GRIA2 / GLUR2 > LS-C344055
 
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Catalog Number Size Price
LS-C344055-10 10 µg $318 
LS-C344055-100 100 µg (0.5 mg/ml) $470 
GRIA2 / GLUR2 Antibody - GRIA2 antibody IHC-paraffin: Rat Brain Tissue.
GRIA2 / GLUR2 Antibody - GRIA2 antibody IHC-paraffin: Mouse Brain Tissue.
GRIA2 / GLUR2 Antibody - IF analysis of GRIA2 using anti-GRIA2 antibody GRIA2 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-GRIA2 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
GRIA2 / GLUR2 Antibody - Western blot analysis of GRIA2 using anti-GRIA2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRIA2 at approximately 110KD. The expected band size for GRIA2 is at 99KD.
GRIA2 / GLUR2 Antibody - GRIA2 antibody Western blot. All lanes: Anti GRIA2 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted band size: 99 kD. Observed band size: 99 kD.
GRIA2 / GLUR2 Antibody - Flow Cytometry analysis of U-87MG cells using anti-GRIA2 antibody. Overlay histogram showing U-87MG cells stained with anti-GRIA2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
GRIA2 / GLUR2 Antibody - GRIA2 antibody IHC-paraffin: Rat Brain Tissue.
GRIA2 / GLUR2 Antibody - GRIA2 antibody IHC-paraffin: Mouse Brain Tissue.
GRIA2 / GLUR2 Antibody - IF analysis of GRIA2 using anti-GRIA2 antibody GRIA2 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-GRIA2 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
GRIA2 / GLUR2 Antibody - Western blot analysis of GRIA2 using anti-GRIA2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRIA2 at approximately 110KD. The expected band size for GRIA2 is at 99KD.
GRIA2 / GLUR2 Antibody - GRIA2 antibody Western blot. All lanes: Anti GRIA2 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted band size: 99 kD. Observed band size: 99 kD.
GRIA2 / GLUR2 Antibody - Flow Cytometry analysis of U-87MG cells using anti-GRIA2 antibody. Overlay histogram showing U-87MG cells stained with anti-GRIA2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human GRIA2 / GLUR2 Antibody (aa25‑360, IHC, WB) LS‑C344055

Polyclonal Rabbit anti‑Human GRIA2 / GLUR2 Antibody (aa25‑360, IHC, WB) LS‑C344055

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Antibody:
GRIA2 / GLUR2 Rabbit anti-Human Polyclonal (aa25-360) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344055-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GRIA2 / GLUR2 Rabbit anti-Human Polyclonal (aa25-360) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
GLUR2 antibody LS-C344055 is an unconjugated rabbit polyclonal antibody to GLUR2 (GRIA2) (aa25-360) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human GRIA2 / GLUR2
Synonyms
GRIA2 | AMPA 2 | GluR-K2 | Glutamate receptor 2 | HBGR2 | GluA2 | GluR-2 | GluR-B | GLUR2 | GLURB
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human GRIA2 recombinant protein (Position: N25-I360). Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA2.
Epitope
aa25-360
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GRIA2 / GLUR2
P42262 NM_000826 NP_000817.2

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Featured Products

GRIA2 / GLUR2 Antibody - Anti-GRIA2 / GLUR2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC‑plus™ GRIA2 / GLUR2 Antibody (aa175‑430) LS‑B2811
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
GRIA2 / GLUR2 Antibody - Immunohistochemistry of paraffin-embedded Human gastric cancer using GRIA2 Polyclonal Antibody at dilution of 1:40.
GRIA2 / GLUR2 Antibody LS‑C402493
Species: Human, Mouse, Rat
Applications: IHC, ELISA
GRIA2 / GLUR2 Antibody - Immunohistochemical analysis of GLUR2 (pS880) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GRIA2 / GLUR2 Antibody (phospho‑Ser880) LS‑C356073
Species: Human, Monkey, Mouse, Rat, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
GRIA2 / GLUR2 Antibody - Western blot of GluR-2 antibody
GRIA2 / GLUR2 Antibody (820‑900 aa) LS‑C383711
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
GRIA2 / GLUR2 Antibody - Immunohistochemistry analysis of paraffin-embedded human brain, using GluR2 (Phospho-Ser880) Antibody. The picture on the right is blocked with the phospho peptide.
GRIA2 / GLUR2 Antibody (phospho‑Ser880) LS‑C117367
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
GRIA2 / GLUR2 ELISA Kit
Human GRIA2 / GLUR2 (Sandwich ELISA) ELISA Kit - LS-F8851
Reactivity: Human
Range: 0.313-20 ng/ml
See all GRIA2 / GLUR2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/20/2024
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