Mouse Gr-1 Antigen

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

RB6-8C5

Unconjugated. Also available conjugated with RPE, Biotin.

Protein G purified

Unmodified

Normal murine bone marrow cells

Recognizes the mouse Gr-1 antigen, a 21-25kD cell surface protein that belongs to the Ly-6 family of proteins. Clone Reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated. The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported. Clone RB6-8C5 has been reported to deplete mouse mature neutrophils in vivo. XXX recommend the use of MCA2387EL for functional studies.

• IHC
• IHC - Frozen
• Immunofluorescence
• Western blot
• Immunoprecipitation
• Flow Cytometry (1:100 - 1:200)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.