Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:2000 - 1:5000)
ELISA (1:2000 - 1:5000)
Recombinant human GPX1.
Recognizes human GPX1. Detects a band of ~23kDa by Western blot.
ELISA - Direct and indirect: 1:2000-1:5000. Western Blot - 1:2000-1:5000 using ECL. suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. suggested incubation time is 1 hour at room temperature.
PBS, pH 7.4, no preservatives.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
GPX1 / Glutathione Peroxidase is a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon.