Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Western blot (1:10000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human glutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Rat, Hamster, Panda, Dog, Horse, Pig (95%); Mouse, Elephant, Zebu, Bovine, Rabbit (90%); Bat, Opossum, Platypus (80%).
Specific for glutathione peroxidase 1 (GPx-1) protein. Species cross-reactivity: Human and rat.
Suitable for use in Western Blot and Immunohistochemistry. Immunohistochemistry (Frozen and Paraffin): 1:2000-1:20000. Western Blot: 1:10000-1:100000. Under reducing conditions recognizes a 22kD protein in human brain tissue and glutathione peroxidase isolated from human erythrocytes.
GPX1 / Glutathione Peroxidase is a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon.