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Catalog Number Size Price
LS-A1865-50 50 µg (1 mg/ml) $440 
GPR80 / GPR99 / OXGR1 Antibody - Anti-OXGR1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
GPR80 / GPR99 / OXGR1 Antibody - Anti-OXGR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
GPR80 / GPR99 / OXGR1 Antibody - Anti-OXGR1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
GPR80 / GPR99 / OXGR1 Antibody - Anti-OXGR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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IHC‑plus™ Polyclonal Rabbit anti‑Human GPR80 / GPR99 / OXGR1 Antibody (Extracellular Domain, IHC) LS‑A1865

IHC‑plus™ Polyclonal Rabbit anti‑Human GPR80 / GPR99 / OXGR1 Antibody (Extracellular Domain, IHC) LS‑A1865

Antibody:
GPR80 / GPR99 / OXGR1 Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-A1865-50

Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GPR80 / GPR99 / OXGR1 Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
OXGR1 antibody LS-A1865 is an unconjugated rabbit polyclonal antibody to human OXGR1 (GPR80 / GPR99) (Extracellular Domain). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human GPR80 / GPR99 / OXGR1
Synonyms
OXGR1 | 2-oxoglutarate receptor 1 | Alpha-ketoglutarate receptor 1 | G protein-coupled receptor 80 | GPR80 | G-protein coupled receptor 99 | GPR99 | p2Y purinoceptor 15 | p2Y-like nucleotide receptor | p2RY15 | Oxoglutarate receptor 1 | p2Y-like GPCR | p2Y15 | G protein-coupled receptor 99 | G-protein coupled receptor 80
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 17 amino acid peptide from 2nd extracellular domain of human OXGR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Monkey, Mouse, Rat, Rabbit (94%); Dog, Bat, Hamster, Panda, Horse (88%).
Epitope
Extracellular Domain
Specificity
Human OXGR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (15 - 20 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR80 / GPR99 / OXGR1
Q96P68 NM_080818 NP_543008.3

Validation

GPR80 / GPR99 / OXGR1 Antibody - Anti-OXGR1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-OXGR1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-OXGR1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

GPR80 / GPR99 / OXGR1 Antibody - Anti-OXGR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-OXGR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-OXGR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ GPR80 / GPR99 / OXGR1 Antibody (Extracellular Domain) for IHC, ELISA LS-A1865 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

alpha -Ketoglutarate regulates acid-base balance through an intrarenal paracrine mechanism. Tokonami N, Morla L, Centeno G, Mordasini D, Ramakrishnan SK, Nikolaeva S, Wagner CA, Bonny O, Houillier P, Doucet A, Firsov D. The Journal of clinical investigation. 2013 Jul;123:3166-71.

Exercise-induced a-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation. Yexian Yuan , Pingwen Xu , Qingyan Jiang , Xingcai Cai, Tao Wang, Wentong Peng, Jiajie Sun, Canjun Zhu, Cha Zhang, Dong Yue, Zhihui He, Jinping Yang, Yuxian Zeng, Man Du, Fenglin Zhang, Lucas Ibrahimi, Sarah Schaul, Yuwei Jiang, Jiqiu Wang, Jia Sun, Qiaoping Wang, Liming Liu, Songbo Wang, Lina Wang, Xiaotong Zhu, Ping Gao, Qianyun Xi, Cong Yin, Fan Li, Guli Xu, Yongliang Zhang, Gang Shu. The EMBO journal. 2020 Apr;39:e103304.

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Requested From: United States
Date Requested: 4/18/2024