CRTH2 antibody LS-A153 is an unconjugated rabbit polyclonal antibody to human CRTH2 (GPR44). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Human GPR44 / CRTH2
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin (15 µg/ml)
- (applications tested for the base form of this product only)
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GPR44 / CRTH2 antibody was raised against synthetic 20 amino acid peptide from C-terminal cytoplasmic domain of human GPR44 / CRTH2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Pig (85%); Elephant (80%).
- Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A153. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human GPR44 / CRTH2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except KCNN2 (50%).
Immunohistochemistry: LS-A153 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A153 was determined to be 15 ug/ml.
PBS, 0.1% Sodium Azide
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
For research use only.