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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPR12 Antibody (C‑Terminus) IHC‑plus™ LS‑A78
GPR12 antibody LS-A78 is an unconjugated rabbit polyclonal antibody to GPR12 from human and monkey. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A78-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment
Description
GPR12 antibody LS-A78 is an unconjugated rabbit polyclonal antibody to GPR12 from human and monkey. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GPR12
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Dog, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2 - 4 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR12 antibody was raised against synthetic 16 amino acid peptide from C-terminus of human GPR12 / GPCR12. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Mouse, Panda, Dog, Pig (94%); Hamster, Elephant, Bat, Horse, Rabbit, Opossum, Turkey (88%); Rat, Bovine, Chicken, Platypus, Xenopus, Stickleback (81%).
Epitope
C-Terminus
Specificity
Human GPR12 / GPCR12. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except REM1 (50%).
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GPR12
P47775 NM_005288 NP_005279.1

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Images

Immunohistochemistry

Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot with GPR12 antibody.  Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.
Western blot with GPR12 antibody. Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.

Immunohistochemistry

Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot with GPR12 antibody.  Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.
Western blot with GPR12 antibody. Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.

Immunohistochemistry

Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot with GPR12 antibody.  Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.
Western blot with GPR12 antibody. Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.

Immunohistochemistry

Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR12 / GPCR12 antibody IHC of human brain, pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot with GPR12 antibody.  Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.
Western blot with GPR12 antibody. Lane 1, liver; lane 2, brain; lane 3, lung; lane 4, kidney; lane 5, spleen; lane 6, testis; lane 7, ovary; lane 8 heart; lane 9, pancreas.

Requested From: United States
Date Requested: 12/9/2018
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