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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C352266-100 100 µl (1 mg/ml) $245 
GPCRW / GPR18 Antibody - Immunofluorescent analysis of GPR18 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GPCRW / GPR18 Antibody - Western blot analysis of GPR18 expression in HEK293T (A); Jurkat (B); mouse brain (C); rat brain (D) whole cell lysates.
GPCRW / GPR18 Antibody - Immunofluorescent analysis of GPR18 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GPCRW / GPR18 Antibody - Western blot analysis of GPR18 expression in HEK293T (A); Jurkat (B); mouse brain (C); rat brain (D) whole cell lysates.
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Polyclonal GPCRW / GPR18 Antibody (Internal, IF, WB) LS‑C352266

Polyclonal GPCRW / GPR18 Antibody (Internal, IF, WB) LS‑C352266

Antibody:
Rabbit Polyclonal to Human GPCRW / GPR18
Application:
ICC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$245 
LS-C352266-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPCRW / GPR18
Application:
ICC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
GPR18 antibody LS-C352266 is an unconjugated rabbit polyclonal antibody to GPR18 (GPCRW ) from human, mouse and rat. Validated for Flow, ICC, IF and WB.
Target
Human GPCRW / GPR18
Synonyms
GPR18 | GPCRW | G protein-coupled receptor 18 | NAGly receptor | G-protein coupled receptor 18 | N-arachidonyl glycine receptor
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Flow Cytometry (1:100 - 1:400)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human GPR18.
Blocking Peptide
LS-E42072 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C352266. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Recognizes endogenous levels of GPR18 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPCRW / GPR18
Q14330 NM_005292 NP_005283.1

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Requested From: United States
Date Requested: 2/18/2020