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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C810245-100 100 µl (1 mg/ml) $325 
LS-C810245-200 200 µl (1 mg/ml) $375 
GPCRW / GPR18 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
GPCRW / GPR18 Antibody - Western blot analysis of extracts of HT29 cells using GPR18 antibody.
GPCRW / GPR18 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
GPCRW / GPR18 Antibody - Western blot analysis of extracts of HT29 cells using GPR18 antibody.
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Polyclonal GPCRW / GPR18 Antibody (IF, WB) LS‑C810245

Polyclonal GPCRW / GPR18 Antibody (IF, WB) LS‑C810245

Antibody:
Rabbit Polyclonal to Human GPCRW / GPR18
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C810245-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPCRW / GPR18
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
GPR18 antibody LS-C810245 is an unconjugated rabbit polyclonal antibody to GPR18 (GPCRW ) from human and mouse. Validated for ELISA, IF and WB.
Target
Human GPCRW / GPR18
Synonyms
GPR18 | GPCRW | G protein-coupled receptor 18 | NAGly receptor | G-protein coupled receptor 18 | N-arachidonyl glycine receptor
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • ELISA (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20 °C for up to 12 months
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPCRW / GPR18
Q14330 NM_005292 NP_005283.1

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Requested From: United States
Date Requested: 2/25/2020