GPR101 antibody LS-A101962 is a PE-conjugated rabbit polyclonal antibody to human GPR101 (GPCR6). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with AP
(This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
GPCR6 / GPR101 antibody was raised against synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR101. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (95%); Bovine, Horse, Pig (89%); Bat, Panda, Rabbit (84%).
Human GPR101. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZBTB10 (47%).
was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1836
was determined to be 7.3 ug/ml.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.