Target
Human GPC6 / Glypican 6
Synonyms
GPC6 | Glypican proteoglycan 6 | OMIMD1 | Glypican 6 | Glypican-6
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived recombinant human Glypican 6 (aa24-527) Accession # NP_005699.
Specificity
Recognizes human Glypican 6.
Applications
- Western blot (1 - 2 µg/ml)
- Flow Cytometry
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Flow Cytometry, Western Blot and ELISA. Western Blot: 1-2 ug/ml Using a colorimetric detection system, the detection limit for rhGlypican 6 is ~10 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 ug/ml The detection limit for rhGlypican 6 is. ~7 ng/well. Flow Cytometry: 0.2 mg/ml. Add 10 ul to 2.5x10^5 cells in a total reaction volume not exceeding 200ul.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200ul sterile PBS.
Storage
Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee