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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPA33 / A33 Antibody (aa264‑293) LS‑C166592
A33 antibody LS-C166592 is an unconjugated rabbit polyclonal antibody to human A33 (GPA33). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C166592-400
400 µl
$305
Description
A33 antibody LS-C166592 is an unconjugated rabbit polyclonal antibody to human A33 (GPA33). Validated for Flow, IHC and WB.
Target
Human GPA33 / A33
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9836 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166592. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa264-293
Specificity
This GPA33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human GPA33.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About GPA33 / A33
Q99795 NM_005814 NP_005805.1

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Images

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.
Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.

Flow Cytometry

GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.
Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.

Flow Cytometry

GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.
Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.

Flow Cytometry

GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
GPA33 Antibody IHC of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.
Western blot of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35 ug/lane). GPA33 (arrow) was detected using the purified antibody.

Flow Cytometry

GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GPA33 Antibody flow cytometry of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/20/2018
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