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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154377-1 1 mg (3.6 mg/ml) $295 
Rat IgG Antibody - Western blot of Goat anti-Rat IgG Antibody. Lane 1: Rat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Rat antibody at 1000 overnight at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:20000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rat IgG. Other band(s): None. This image was taken for the unmodified form of this product. Other forms have not been tested.

Goat Anti‑Rat IgG Secondary Antibody (Fab'2) LS‑C154377

Goat Anti‑Rat IgG Secondary Antibody (Fab'2) LS‑C154377

Antibody:
Goat Polyclonal to Rat Rat IgG
Application:
IHC, WB, Flo, ELISA
Reactivity:
Rat
Format:
Unconjugated, Fab'2
Price
Catalog Number
$295 
LS-C154377-1
1 mg
3.6 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Rat Rat IgG
Application:
IHC, WB, Flo, ELISA
Reactivity:
Rat
Format:
Unconjugated, Fab'2

Specifications

Description
Rat IgG antibody LS-C154377 is an unconjugated goat polyclonal antibody. Validated for ELISA, Flow, IHC and WB.
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Fab'2
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-Rat IgG was produced by repeated immunization with Rat IgG whole molecule in goat.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Mouse Serum Proteins.
Usage
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/20/2019