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Catalog Number Size Price
LS-C745599-10 10 mg (10.2 mg/ml) $1,017 
Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (GOAT) antibody Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule Lane 2: Rat IgG F(c) Fragment Lane 3: Rat IgG Fab Fragment Lane 4: Rat IgM Whole Molecule Lane 5: Rat Serum All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.

Polyclonal Goat Anti‑Rat IgG Secondary Antibody (IHC, WB) LS‑C745599

Polyclonal Goat Anti‑Rat IgG Secondary Antibody (IHC, WB) LS‑C745599

Antibody:
Rat IgG Goat anti-Rat Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$1,017
LS-C745599-10
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rat IgG Goat anti-Rat Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Rat IgG antibody LS-C745599 is an unconjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Mouse Serum Proteins. Specificity was confirmed by ELISA at less than 1% reactivity.
Applications
  • IHC (1:5000 - 1:20000)
  • Western blot (1:10000 - 1:40000)
  • ELISA (1:20000 - 1:110000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021