(tested or 100% immunogen sequence identity)
- IHC (1:500 - 1:5000)
- Western blot (1:20000)
- ELISA (1:20000)
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Rat IgG Fc antibody was raised against rat IgG F(c) whole molecule. Label: Horseradish Peroxidase (HRP).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F (ab')2 or Bovine, Horse, Human Serum Proteins.
Suitable for Western Blot, ELISA, Immunohistochemistry and Immunoperoxidase electron microscopy. ELISA: 1:20000 to 1:200000 using ABTS as a substrate for 30 minutes at room temperature. Western Blot: 1:20000 to 1:200000. Immunohistochemistry: 1:500-1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% Gentamicin sulfate. Reconstitution: Reconstitute, 500 ul sterile dH2O.
500 µl Reconstitute with ddH2O.
For research use only.
This antibody carries the LSBio 100% Guarantee.