Reactivity
Rat
(tested or 100% immunogen sequence identity)
Purification
Immunoaffinity purified
Immunogen
Rat IgA a-chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgG or Rat M. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.
Applications
- Immunofluorescence
- Flow Cytometry
Usage
Suitable for use in Immunomicroscopy, Flow Cytometry and FACS. Optimal dilutions have not been tested. Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons). Absorption Wavelength: 550nm. Emission Wavelength: 570nm. Fluorochrome/Protein Ratio: 2.5 moles TRITC per mole of Goat IgG. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized 10 mg/ml BSA (IgG, Protease free), PBS, pH 7.2, 0.01% Sodium Azide
Reconstitution
Sterile 40-50% glycerol.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee