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Goat Anti‑Rabbit IgG Secondary Antibody (PE) LS‑C60868

Catalog Number Size Price
LS-C60868-1 1 ml (0.5 mg/ml) $335 
Antibody

Goat Anti‑Rabbit IgG Secondary Antibody (PE) LS‑C60868

Antibody:
Goat Polyclonal to Rabbit Rabbit IgG
Application:
IF, Flo
Reactivity:
Rabbit
Format:
Phycoerythrin, Unmodified
Price
Catalog Number
$335 
LS-C60868-1
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Rabbit Rabbit IgG
Application:
IF, Flo
Reactivity:
Rabbit
Format:
Phycoerythrin, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C60868 is a PE-conjugated goat polyclonal antibody. Validated for Flow and IF.
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Phycoerythrin
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100 - 1:250)
  • Flow Cytometry (1:100 - 1:250)
Immunogen
Rabbit IgG whole molecule
Specificity
anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins
Usage
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Pre-Conjugation Min ELISA Endpoint Dilution: 1:10000 Actual: 1:104000 Disposition: PASS. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mg/ml BSA (IgG, Protease free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
1 ml deionized water
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Species: Rabbit
Applications: IHC, Western blot, ELISA
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Species: Rabbit
Applications: Immunofluorescence
HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated  (Min X Human Serum Proteins) lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA
RABBIT IgG (H&L) Antibody Alkaline Phosphatase Conjugated Western Blot. Western Blot of Goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Lane 1: Rabbit IgG Load: 100 ng per lane Secondary antibody: RABBIT IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kD, 55 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA
Product
Species: Rabbit
Applications: IHC - Frozen, Western blot, ELISA
Reactivity: Rabbit
Range: 0.1-100 ng/ml

Requested From: United States
Date Requested: 9/15/2019