(tested or 100% immunogen sequence identity)
- Immunofluorescence (1:100 - 1:250)
- Western blot
- Flow Cytometry (1:100 - 1:250)
Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in goat.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Pre-Conjugation Min ELISA Endpoint Dilution: 1:10000 Actual: 1:104000 Disposition: PASS. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitute in 1 mL of deionized water.
Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark.
For research use only. Intended for use by laboratory professionals.
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