LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60865
Rabbit IgG antibody LS-C60865 is an HRP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C60865-1000
1000 µg
Unavailable

Popular Rabbit IgG Products

Antibody
Species: Rabbit
Applications: IHC, IHC - Frozen, Western blot, ELISA
Antibody
Species: Rabbit
Applications: Immunofluorescence, Flow Cytometry
Antibody
Species: Rabbit
Applications: Immunofluorescence
RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA
HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (LS-C60887 lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rabbit
Applications: IHC, Western blot, ELISA

Product Description

Rabbit IgG antibody LS-C60865 is an HRP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
About Rabbit IgG
IgG is the most common type of antibody found in the circulation, representing approximately 75% of serum antibodies. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape.

Specifications

Target
Rabbit IgG
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Rabbit IgG antibody was raised against rabbit IgG whole molecule.
Specificity
anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Hamster, Human, Mouse, Rat and Sheep Serum Proteins
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% Gentamicin.
Reconstitution
1 ml deionized water
Storage
Store at 4°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.
HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Dot Blot

secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.
secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.
HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Dot Blot

secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.
secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.
HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Dot Blot

secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.
secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.
HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Dot Blot

secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.
secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number