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Goat Anti-Rabbit IgG Secondary Antibody (HRP) LS-C60865

Ordering

Wt. Vol. Conc. Price
1000 µg 1 ml - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Rabbit IgG Antibodies

Goat Anti-Rabbit IgG Secondary Antibody (PE) LS-C60868
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Goat Anti-Rabbit IgG Secondary Antibody (HRP) LS-C60884
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Western blot Image
Goat Anti-Rabbit IgG Secondary Antibody (HRP) LS-C60887
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Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Rabbit IgG
Rabbit
IHC, Western blot, ELISA
HRP Conjugated

Details

Rabbit IgG
Goat
Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Horseradish Peroxidase
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA

Specificity and Use

Rabbit IgG antibody was raised against rabbit IgG whole molecule.
anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Hamster, Human, Mouse, Rat and Sheep Serum Proteins
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% Gentamicin.
1 ml deionized water
Store at 4°C.
For research use only.

About Rabbit IgG

IgG is the most common type of antibody found in the circulation, representing approximately 75% of serum antibodies. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape.

Western blot

Western blot
HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Dot Blot

Dot Blot
secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (LS-C60865) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/8/2016

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