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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C153940-500 500 µg (1 mg/ml) $395 
Rabbit IgG Antibody - ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Goat Anti‑Rabbit IgG Secondary Antibody (Atto 647N) LS‑C153940

Goat Anti‑Rabbit IgG Secondary Antibody (Atto 647N) LS‑C153940

Antibody:
Goat Polyclonal to Rabbit Rabbit IgG
Application:
IF, WB, ELISA
Reactivity:
Rabbit
Format:
Atto 647N, Unmodified
Price
Catalog Number
$395 
LS-C153940-500
500 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Rabbit Rabbit IgG
Application:
IF, WB, ELISA
Reactivity:
Rabbit
Format:
Atto 647N, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C153940 is an Atto 647N-conjugated goat polyclonal antibody. Validated for ELISA, IF and WB.
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 647N
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • ELISA (1:20000)
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Usage
The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/20/2019