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Antibodies > Rabbit IgG > LS-C745593
 
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Catalog Number Size Price
LS-C745593-100 100 µg (1 mg/ml) $310 
Rabbit IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Rabbit IgG Antibody - Western Blot of Anti-Rabbit IgG (H&L) (GOAT) antibody Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule Lane 2: Rabbit IgG F(ab) Fragment Lane 3: Rabbit IgG F(c) Fragment Lane 4: Rabbit IgM Whole Molecule Lane 5: Normal Rabbit Serum All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Rabbit IgG Antibody - Western Blot of Goat anti-Rabbit IgG Pre-Adsorbed Atto 488 Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Atto 488 goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
Rabbit IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Rabbit IgG Antibody - Western Blot of Anti-Rabbit IgG (H&L) (GOAT) antibody Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule Lane 2: Rabbit IgG F(ab) Fragment Lane 3: Rabbit IgG F(c) Fragment Lane 4: Rabbit IgM Whole Molecule Lane 5: Normal Rabbit Serum All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Rabbit IgG Antibody - Western Blot of Goat anti-Rabbit IgG Pre-Adsorbed Atto 488 Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Atto 488 goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
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Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (Atto 488, IF, WB) LS‑C745593

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (Atto 488, IF, WB) LS‑C745593

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Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (Atto 488) Antibody
Application:
IF, WB, FLISA
Reactivity:
Rabbit
Format:
Atto 488, Unmodified
Price
Catalog Number
$310
LS-C745593-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (Atto 488) Antibody
Application:
IF, WB, FLISA
Reactivity:
Rabbit
Format:
Atto 488, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C745593 is an Atto 488-conjugated goat polyclonal antibody. Validated for FLISA, IF and WB.
Target
Rabbit IgG
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 488
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60884
Species: Rabbit
Applications: IHC, Western blot, ELISA
Rabbit IgG Antibody - Western Blot of Fluorescein conjugated Goat anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.
Goat Anti‑Rabbit IgG Secondary Antibody (FITC) LS‑C60881
Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60887
Species: Rabbit
Applications: IHC, Western blot, ELISA, Dot Blot, Electron Microscopy
Product
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C56309
Species: Rabbit
Applications: IHC - Frozen, Western blot, ELISA
Rabbit IgG Antibody - Western blot of Peroxidase conjugated Rat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
Rat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60921
Species: Rabbit
Applications: IHC, Western blot, ELISA
See all Rabbit IgG Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
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