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Catalog Number Size Price
LS-C745592-100 100 µg (1 mg/ml) $275 
Rabbit IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells) stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Rabbit IgG Antibody - ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 µg/mL final concentration of anti-a-tubulin and detected with 0.2 µg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] secondary antibody. Also shown in this 2-color STED image is Anti-HDAC-1 [RABBIT]
Rabbit IgG Antibody - Western Blot of ATTO 425 conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 425 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa/55 kDa for Rabbit IgG. Other band(s): none.
Rabbit IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells) stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Rabbit IgG Antibody - ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 µg/mL final concentration of anti-a-tubulin and detected with 0.2 µg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] secondary antibody. Also shown in this 2-color STED image is Anti-HDAC-1 [RABBIT]
Rabbit IgG Antibody - Western Blot of ATTO 425 conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 425 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa/55 kDa for Rabbit IgG. Other band(s): none.
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Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (Atto 425, IF, WB) LS‑C745592

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (Atto 425, IF, WB) LS‑C745592

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (Atto 425) Antibody
Application:
IF, WB, FLISA
Reactivity:
Rabbit
Format:
Atto 425, Unmodified
Price
Catalog Number
$275
LS-C745592-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (Atto 425) Antibody
Application:
IF, WB, FLISA
Reactivity:
Rabbit
Format:
Atto 425, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C745592 is an Atto 425-conjugated goat polyclonal antibody. Validated for FLISA, IF and WB.
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 425
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021