(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:600)
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Rabbit IgG Fc antibody was raised against rabbit IgG F (ab')2 fragment.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F (ab')2 or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Suitable for use in Western Blot (Western or Dot Blot), ELISA and Immunohistochemistry. ELISA: 1:600 to 1:2800. 1.0 ug of rabbit IgG was used in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M Magnesium chloride, 0.0001 M Zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.
Store at 4°C. Do not freeze.
For research use only.
This antibody carries the LSBio 100% Guarantee.