(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:5000)
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Mouse IgG Fab'2 antibody was raised against mouse IgG F (ab')2 fragment.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG, Mouse IgG F (ab')2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Suitable for use in Western Blot (Western or Dot Blot), ELISA and Immunohistochemistry. ELISA: 1:5000 to 1:25000. 1.0 ug of mouse IgG was used in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M Magnesium chloride, 0.0001 M Zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.
Store at 4°C. Do not freeze.
For research use only.
This antibody carries the LSBio 100% Guarantee.