Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
Specificity and Use
Mouse IgD antibody was raised against mouse IgD
This polyclonal goat anti-mouse IgD antiserum has been tested for in vivo activation of mouse B cells, as measured by induction of IgE^1 and IL-4 (Th2)^2 responses. A dose of 200 ul per mouse is suggested for induction of IgE responses, administered by subcutaneous or intraperitoneal injection. High levels of IgE were measurable by ELISA within 5-7 days of administration. High levels of IL-4 were measurable by in vivo cytokine capture assay within 5 days of administration. Intravenous injection is not recommended. Induction of IgE In vivo: Treatment of 3 mice with 0.2 ml each of the goat anti-mouse IgD antiserum yielded circulating IgE levels of 2.7 ug/ml, 1.9 ug/ml, and 1.3 ug/ml within 8 days; untreated control mice were found to have less than 76 ng/ml circulating IgE. Induction of IL-4 In vivo: Treatment of 5 mice with 0.2 ml each of saline control or the goat anti-mouse IgD antiserum yielded IL-4 levels of 96 +/-1.23 pg/ml (geometric mean and standard error) and 3583 +/- 1.03 pg/ml, respectively, as measured by in vivo IL-4 capture assay.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.