Human IgM

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

FITC

Affinity chromatography

Unmodified

Human IgM whole molecule

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain proteins.

• Immunofluorescence (1:1000 - 1:5000)
• Flow Cytometry (1:500 - 1:2500)
• Fluorophore-Linked Immunosorbent Assay (1:10000 - 1:50000)

This product is designed for use as a positive control for anti-epitope tag antibodies. This E. coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: GST--T7--HSV--cMyc--VSV--GluGlu--V5--E-tag--Flag--S tag--HA--6XHis. A 41 kD recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E. coli lysate was prepared by lysing cells in loading buffer, heating to 95? C for 10' followed by centrifugation at 13000 g for 15'. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 1 mL of deionized water.

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.