Human IgM Fc

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Tetramethyl Rhodamine Iso-Thiocyanate

Purified

Fab'2

Purified IgM prepared from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Tetramethylrhodamine isothiocyanate-conjugated F(ab')2 fragments of polyclonal Goat IgG antibodies to Human IgM, Fc specific.

• IHC
• IHC - Frozen
• ICC
• Immunofluorescence
• ELISA

The antiserum is reacting with to the Fc subunits of IgM. It does not react with other immunoglobulins or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgM. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.