Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC (1:1000 - 1:5000)
Western blot (1:2000 - 1:10000)
ELISA (1:20000 - 1:100000)
Specificity and Use
Human IgG Fc antibody was raised against human IgG Fc fragment.
anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab')2 or Bovine, Horse, Mouse and Rat Serum Proteins
Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
1 ml deionized water
Store lyophilized at 4°C. Once reconstituted, aliquot and store at 20°C. Avoid freeze-thaw cycles.
For research use only.
About Human IgG Fc
The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system.
Western Blot - F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed. Western Blot of Goat anti-F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed Lane 1: Human Fc Load: 100 ng per lane Primary Antibody: F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed Size: 28 kD/28 kD.