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| Catalog Number | Size | Price |
|---|---|---|
| LS-C346510-1 | 1 ml | $520 |
Polyclonal Goat Anti‑Guinea Pig IgG Secondary Antibody (HRP, IHC) LS‑C346510
Polyclonal Goat Anti‑Guinea Pig IgG Secondary Antibody (HRP, IHC) LS‑C346510
Antibody:
Guinea Pig IgG Goat anti-Guinea pig Polyclonal (HRP) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Guinea pig
Format:
Horseradish Peroxidase, Unmodified
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Guinea Pig IgG Goat anti-Guinea pig Polyclonal (HRP) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Guinea pig
Format:
Horseradish Peroxidase, Unmodified
Other formats:
Specifications
Description
Guinea Pig IgG antibody LS-C346510 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, ICC and IHC.
Host
Goat
Reactivity
Guinea pig
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Pools of purified polyclonal IgG1 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Guinea Pig IgG1, subclass specific.
Applications
- IHC
- IHC - Paraffin
- ICC
- ELISA
- Dot Blot
- Applications tested for the base form of this product only
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the subclass IgG1. It does not react with the other subclass of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgG1 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2000 depending on the method used.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with 1ml distilled H2O.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Customer Reviews (0)
Featured Products
Species:
Guinea pig
Applications:
Immunofluorescence, Western blot, Flow Cytometry, Fluorophore-Linked Immunosorbent Assay
Species:
Guinea pig
Applications:
IHC, Western blot, ELISA
Species:
Guinea pig
Applications:
IHC, Western blot, ELISA
Species:
Guinea pig
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
