Clonality
IgG F(ab')2
Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Applications
- IHC (1:500 - 1:1000)
- Western blot (1:1000 - 1:5000)
- ELISA (1:1000 - 1:5000)
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Usage
This product has been assayed against 1.0 ug of Biotinylated BSA in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:5000 is suggested for this product. This product has been assayed against 1.0 ug of Biotinylated BSA in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:5000 is suggested for this product. This product has been assayed against 1.0 ug of Biotinylated BSA in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:5000 is suggested for this product. This product has been assayed against 1.0 ug of Biotinylated BSA in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:5000 is suggested for this product. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee