Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Biotinylated IgG. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
This product has been assayed against 1.0 ug of Biotin conjugated IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2000 of the reconstitution concentration is suggested for this product.
0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50% glycerol, 1% BSA, 0.01% sodium azide, sterile filtered
Store at 4°C. Do not freeze.
For research use only.
This antibody carries the LSBio 100% Guarantee.