Bovine GNAS

Rabbit

Bovine, Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish, Drosophila, Insect (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

Synthetic peptide corresponding to the C-Terminus of bovine Gs alpha (RMHLRQYELL) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

aa1028-1037

Recognizes both the large (MW 48-52kD) and small (MW43-45kD) forms of bovine Gs alpha. Does not cross-react with other G proteins.

• ICC (1:100)
• Western blot (1:500 - 1:1000)
• Immunoprecipitation

Western Blot: 1:1000-1:2000 detected both forms of Gs alpha as a doublet in 20ug of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-C24097 (1:1000). Proteins were visualized using a IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.Immunoprecipitation: 2ul immunoprecipitates Gs alpha(45kD) from 500ug of solubilized CHO and mouse brain membrane preparations.Immunocytochemistry: 1:200 detected Gs alpha in 3T3 cells fixed for 3 minutes with 100% ice-cold acetone.Optimal dilutions to be determined by researcher.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q5JWF2 NCBI: NM_080425 NP_536350.2