Glutamate antibody was raised against glutamate-glutaraldehyde.
Recognizes Glutamate (L-Glutamate). Calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (<1:100) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arginine, D/L-aspartate, L-citrulline, L-cysteine, D/L glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:8) against free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue).
Suitable for use in ELISA, Immunohistochemistry. Immunohistochemistry: silver-intensified immunogold or fluorescence. Samples should be fixed with 0.1-2.5% glutaraldehyde for optimum detection. This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1 M phosphate buffer/3% sucrose fixative). The minimum glutaraldehyde concentration is 0.00%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
100 mM phosphate buffer, 1% sterile goat serum, 0.05% Thimerosal, 40% glycerol.
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