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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLUD1/Glutamate Dehydrogenase Antibody (HRP) LS‑C209656
Glutamate Dehydrogenase antibody LS-C209656 is an HRP-conjugated rabbit polyclonal antibody to bovine Glutamate Dehydrogenase (GLUD1). Validated for ELISA, IP and WB.
Antibody
Catalog
Size
Price
LS-C209656-100
100 µg
$425

Popular GLUD1/Glutamate Dehydrogenase Products

Anti-GLUD1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Bovine, Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Species: Human, Monkey, Mouse, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemical analysis of GLUD1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Bovine
Applications: Western blot, ELISA

Product Description

Glutamate Dehydrogenase antibody LS-C209656 is an HRP-conjugated rabbit polyclonal antibody to bovine Glutamate Dehydrogenase (GLUD1). Validated for ELISA, IP and WB.

Specifications

Target
Bovine GLUD1/Glutamate Dehydrogenase
Synonyms
GLUD1, GDH1, GDH 1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 2, GDH, GLUD
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Immunogen
Glutamate Dehydrogenase [Bovine Liver].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Usage
Anti-Glutamate dehydrogenase has been assayed against 1.0 ug of Glutamate Dehydrogenase [Bovine Liver] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:20000 to 1:100000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA
Reconstitution
100 µl Distilled water
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only.
About GLUD1/Glutamate Dehydrogenase
GLUD1/Glutamate Dehydrogenase is glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. P00367 NM_005271 NP_005262.1


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Requested From: United States
Date Requested: 11/13/2018

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