Glutamate Dehydrogenase antibody LS-C59301 is an unconjugated rabbit polyclonal antibody to bovine Glutamate Dehydrogenase (GLUD1). Validated for ELISA and WB.
Bovine GLUD1/Glutamate Dehydrogenase
(tested or 100% immunogen sequence identity)
Delipidated and defibrinated
- Western blot (1:1000 - 1:3000)
- ELISA (1:4000 - 1:16000)
Full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kD in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
For research use only.
About GLUD1/Glutamate Dehydrogenase