LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
GLUD1/Glutamate Dehydrogenase Antibody LS‑C59301
Glutamate Dehydrogenase antibody LS-C59301 is an unconjugated rabbit polyclonal antibody to bovine Glutamate Dehydrogenase (GLUD1). Validated for ELISA and WB.
Catalog
Size
Price
LS-C59301-2000
2000 µl (85 mg/ml)
$1,140

Popular GLUD1/Glutamate Dehydrogenase Products

Anti-GLUD1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Bovine, Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Species: Human, Monkey, Mouse, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemical analysis of GLUD1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Bovine
Applications: Western blot, ELISA

Product Description

Glutamate Dehydrogenase antibody LS-C59301 is an unconjugated rabbit polyclonal antibody to bovine Glutamate Dehydrogenase (GLUD1). Validated for ELISA and WB.

Specifications

Target
Bovine GLUD1/Glutamate Dehydrogenase
Synonyms
GLUD1, GDH1, GDH 1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 2, GDH, GLUD
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:3000)
  • ELISA (1:4000 - 1:16000)
Immunogen
Full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Specificity
Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined
Usage
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kD in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C.
Restrictions
For research use only.
About GLUD1/Glutamate Dehydrogenase
GLUD1/Glutamate Dehydrogenase is glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. P00367 NM_005271 NP_005262.1


Publications (0)


Customer Reviews (0)


Images

Western blot

Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 11/15/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy