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Catalog Number Size Price
LS-C59301-2 2 ml (85 mg/ml) $964 
GLUD1/Glutamate Dehydrogenase Antibody - Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Bovine GLUD1/Glutamate Dehydrogenase Antibody (WB) LS‑C59301

Polyclonal Rabbit anti‑Bovine GLUD1/Glutamate Dehydrogenase Antibody (WB) LS‑C59301

Antibody:
GLUD1/Glutamate Dehydrogenase Rabbit anti-Bovine Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$964
LS-C59301-2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GLUD1/Glutamate Dehydrogenase Rabbit anti-Bovine Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
Glutamate Dehydrogenase antibody LS-C59301 is an unconjugated rabbit polyclonal antibody to bovine Glutamate Dehydrogenase (GLUD1). Validated for ELISA, IP and WB.
Target
Bovine GLUD1/Glutamate Dehydrogenase
Synonyms
GLUD1 | GDH1 | GDH 1 | Glutamate dehydrogenase 1 | Glutamate dehydrogenase 2 | GDH | GLUD
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Applications
  • Western blot (1:1000 - 1:3000)
  • Immunoprecipitation (1:100)
  • ELISA (1:4000 - 1:16000)
Usage
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kD in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GLUD1/Glutamate Dehydrogenase
P00367 NM_005271 NP_005262.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/16/2022