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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLUD1/Glutamate Dehydrogenase Antibody (aa71‑120) LS‑C111057
Glutamate Dehydrogenase antibody LS-C111057 is an unconjugated rabbit polyclonal antibody to Glutamate Dehydrogenase (GLUD1) from human, monkey and zebrafish. Validated for WB.
Catalog
Size
Price
LS-C111057-50
50 µg
$365
Description
Glutamate Dehydrogenase antibody LS-C111057 is an unconjugated rabbit polyclonal antibody to Glutamate Dehydrogenase (GLUD1) from human, monkey and zebrafish. Validated for WB.
Target
Human GLUD1/Glutamate Dehydrogenase
Host
Rabbit
Reactivity
Human, Monkey, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa71-120 of human GLUD1 (P00367, NP_005262). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%); Mouse, Horse, Pig, Opossum, Xenopus (92%); Orangutan, Rat, Bovine (85%); Chicken (84%); Trout, Salmon, Zebrafish (83%).
Blocking Peptide
LS-E16689 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111057. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa71-120
Specificity
Human GLUD1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GLUD1/Glutamate Dehydrogenase
P00367 NM_005271 NP_005262.1

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Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Immunohistochemical analysis of GLUD1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Antibody
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Applications: Western blot, ELISA

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Images

Western blot

GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLUD1 antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/21/2018
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