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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLO1 / Glyoxalase I Antibody LS‑C482325
Glyoxalase I antibody LS-C482325 is an unconjugated rabbit polyclonal antibody to Glyoxalase I (GLO1) from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C482325-50
50 µl (1 mg/ml)
$255
LS-C482325-100
100 µl (1 mg/ml)
$295
LS-C482325-200
200 µl (1 mg/ml)
$375

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Western blot of recombinant GLO1 / Glyoxalase I.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Immunohistochemistry of paraffin-embedded mouse kidney tissue.
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Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Glyoxalase I antibody LS-C482325 is an unconjugated rabbit polyclonal antibody to Glyoxalase I (GLO1) from human, mouse and rat. Validated for IHC and WB.
About GLO1 / Glyoxalase I
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis. Q04760 NM_006708 NP_006699.2

GLO1 Antibody, Aldoketomutase Antibody, Glx I Antibody, Glyoxalase domain containing 1 Antibody, Glyoxalase I Antibody, Lactoyl glutathione lyase Antibody, Lactoylglutathione lyase Antibody, GLOD1 Antibody, Methylglyoxalase Antibody, Ketone-aldehyde mutase Antibody, GLYI Antibody


Specifications

Target
Human GLO1 / Glyoxalase I
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant full length human Glyoxalase I.
Specificity
Recognizes endogenous levels of Glyoxalase I protein.
Presentation
PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.
Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.
Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.
Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.
Western blot analysis of Glyoxalase I expression in 22RV1 (A); PC3 (B); mouse kidney (C) whole cell lysates.

Requested From: United States
Date Requested: 7/21/2018

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