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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLO1 / Glyoxalase I Antibody (clone Glo1a, Biotin) LS‑C142668
Glyoxalase I antibody LS-C142668 is a biotin-conjugated mouse monoclonal antibody to human Glyoxalase I (GLO1). Validated for ELISA, ICC, IF and WB.
Catalog
Size
Price
LS-C142668-100
100 µl
$365

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Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Glyoxalase I antibody LS-C142668 is a biotin-conjugated mouse monoclonal antibody to human Glyoxalase I (GLO1). Validated for ELISA, ICC, IF and WB.
About GLO1 / Glyoxalase I
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis. Q04760 NM_006708 NP_006699.2

GLO1 Antibody, Aldoketomutase Antibody, Glx I Antibody, Glyoxalase domain containing 1 Antibody, Glyoxalase I Antibody, Lactoyl glutathione lyase Antibody, Lactoylglutathione lyase Antibody, GLOD1 Antibody, Methylglyoxalase Antibody, Ketone-aldehyde mutase Antibody, GLYI Antibody


Specifications

Target
Human GLO1 / Glyoxalase I
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [Glo1a]
Conjugations
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.5 µg/ml)
  • ELISA (10 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Full length human GLO1 fusion protein [Swiss-Prot #Q04760]
Specificity
GLO1 (Glo1a).
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunocytochemistry

GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLO1 Antibody - Western Blot on HeLa whole cell extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody - Western Blot on HeLa whole cell extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLO1 Antibody - Western Blot on HeLa whole cell extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody - Western Blot on HeLa whole cell extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLO1 Antibody - Western Blot on HeLa whole cell extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody - Western Blot on HeLa whole cell extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody (Glo1a) - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLO1 Antibody - Western Blot on HeLa whole cell extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 Antibody - Western Blot on HeLa whole cell extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 7/21/2018

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