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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLO1 / Glyoxalase I Antibody (aa35‑84) LS‑C107742
Glyoxalase I antibody LS-C107742 is an unconjugated rabbit polyclonal antibody to Glyoxalase I (GLO1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C107742-50
50 µg
$365

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Product Description

Glyoxalase I antibody LS-C107742 is an unconjugated rabbit polyclonal antibody to Glyoxalase I (GLO1) from human, mouse, rat and other species. Validated for WB.
About GLO1 / Glyoxalase I
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis. Q04760 NM_006708 NP_006699.2

GLO1 Antibody, Aldoketomutase Antibody, Glx I Antibody, Glyoxalase domain containing 1 Antibody, Glyoxalase I Antibody, Lactoyl glutathione lyase Antibody, Lactoylglutathione lyase Antibody, GLOD1 Antibody, Methylglyoxalase Antibody, Ketone-aldehyde mutase Antibody, GLYI Antibody


Specifications

Target
Human GLO1 / Glyoxalase I
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Guinea pig, Chicken, Zebrafish, Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa35-84 of human GLO1 (Q04760, NP_006699). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%); Elephant, Dog, Bovine, Rabbit, Horse, Pig (92%); Xenopus, Smelt, Sablefish, Zebrafish, Grape, Arabidopsis (85%).
Blocking Peptide
LS-E19757 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107742. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human GLO1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLO1 / Glyoxalase I antibody LS-C107742 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/20/2018

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