Glyoxalase I antibody LS-C107742 is an unconjugated rabbit polyclonal antibody to Glyoxalase I (GLO1) from human, mouse, rat and other species. Validated for WB.
Human GLO1 / Glyoxalase I
Human, Monkey, Mouse, Rat, Guinea pig, Chicken, Zebrafish, Yeast
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Synthetic peptide located between aa35-84 of human GLO1 (Q04760, NP_006699). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%); Elephant, Dog, Bovine, Rabbit, Horse, Pig (92%); Xenopus, Smelt, Sablefish, Zebrafish, Grape, Arabidopsis (85%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
About GLO1 / Glyoxalase I