Human GLO1 / Glyoxalase I
Human, Monkey, Mouse, Rat, Guinea pig, Chicken, Zebrafish, Yeast
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Synthetic peptide located between aa35-84 of human GLO1 (Q04760, NP_006699). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%); Elephant, Dog, Bovine, Rabbit, Horse, Pig (92%); Xenopus, Smelt, Sablefish, Zebrafish, Grape, Arabidopsis (85%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.