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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLO1 / Glyoxalase I Antibody (aa1‑184) LS‑C379573
Glyoxalase I antibody LS-C379573 is an unconjugated rabbit polyclonal antibody to human Glyoxalase I (GLO1). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C379573-50
50 µl
$285
LS-C379573-100
100 µl
$385

Popular GLO1 / Glyoxalase I Products

Western blot of recombinant GLO1 / Glyoxalase I.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

Glyoxalase I antibody LS-C379573 is an unconjugated rabbit polyclonal antibody to human Glyoxalase I (GLO1). Validated for ELISA, IHC and WB.

Specifications

Target
Human GLO1 / Glyoxalase I
Synonyms
GLO1, Aldoketomutase, Glx I, Glyoxalase domain containing 1, Glyoxalase I, Lactoyl glutathione lyase, Lactoylglutathione lyase, GLOD1, Methylglyoxalase, Ketone-aldehyde mutase, GLYI
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:20 - 1:200)
  • Western blot (1:200 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Lactoylglutathione lyase protein (aa1-184).
Epitope
aa1-184
Specificity
Human GLO1 / Glyoxalase I
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GLO1 / Glyoxalase I
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis. Q04760 NM_006708 NP_006699.2


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Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.

Western blot

Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.
Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.

Western blot

Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.
Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.

Western blot

Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.
Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human prostate tissue using antibody at 1:100 dilution.

Western blot

Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.
Western blot. All lanes: GLO1 antibody at 2 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 21 kDa. Observed band size: 21 kDa.

Requested From: United States
Date Requested: 11/16/2018

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