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Catalog Number Size Price
LS-C36438-100 100 µg $806 
Antibody

Polyclonal Goat anti‑Human GLI3 Antibody (WB) LS‑C36438

Polyclonal Goat anti‑Human GLI3 Antibody (WB) LS‑C36438

Antibody:
GLI3 Goat anti-Human Polyclonal Antibody
Application:
ICC, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$806
LS-C36438-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
GLI3 Goat anti-Human Polyclonal Antibody
Application:
ICC, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
GLI3 antibody LS-C36438 is an unconjugated goat polyclonal antibody to GLI3 from human. It is reactive with human and mouse. Validated for ELISA, ICC and WB.
Target
Human GLI3
Synonyms
GLI3 | ACLS | GCPS | GLI-Kruppel family member GLI3 | GLI3 full length protein | GLI3-190 | GLI family zinc finger 3 | Gli-3 | GLI3FL | PAP-A | PAPA1 | PHS | PPDIV | Transcriptional activator GLI3 | PAPA | PAPB | GLI3 form of 190 kDa | Oncogene GLI3 | Zinc finger protein GLI3
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rhGLI-3.
Specificity
Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1% cross-reactivity with rhGLI-1 and rhGLI-2.
Applications
  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 ug/ml. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 ug/ml detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 ug/ml. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well.
Presentation
Lyophilized
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GLI3
P10071 NM_000168 NP_000159.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/24/2021