Target
Human GLI / GLI1
Synonyms
GLI1 | Gli-1 | GLI family zinc finger 1 | Glioma-associated oncogene | Glioma-associated oncogene 1 | Oncogene GLI | GLI | Zinc finger protein GLI1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Human, Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogen
Peptide with sequence TYSQCPRLEHYGQ, from the internal region of the protein sequence according to NP_005260.1; NP_001153517.1; NP_001161081.1.
Applications
- Immunofluorescence (10 µg/ml)
- Flow Cytometry (10 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Presentation
Tris saline 0.5 mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee