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Antibodies > GLI / GLI1 > LS-C343972
 
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Catalog Number Size Price
LS-C343972-100 100 µg (0.5 mg/ml) $431 
GLI / GLI1 Antibody - Gli1 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
GLI / GLI1 Antibody - IF analysis of GLI1 using anti-GLI1 antibody GLI1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-GLI1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
GLI / GLI1 Antibody - Western blot analysis of GLI2 using anti-GLI2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: U87 whole cell lysate, lane 2: MCF-7 whole cell lysate, lane 3: HELA whole cell lysate, lane 4: SKOV whole cell lysate, lane 5: HT1080 whole cell lysate, lane 6: COLO320 whole cell lysate, lane 7: HEPG2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLI2 at approximately 118KD. The expected band size for GLI2 is at 118KD.
GLI / GLI1 Antibody - Gli1 antibody Western blot. All lanes: Anti GLI1 at 0.5 ug/ml. WB: Recombinant Human GLI1 Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.
GLI / GLI1 Antibody - Gli1 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
GLI / GLI1 Antibody - IF analysis of GLI1 using anti-GLI1 antibody GLI1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-GLI1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
GLI / GLI1 Antibody - Western blot analysis of GLI2 using anti-GLI2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: U87 whole cell lysate, lane 2: MCF-7 whole cell lysate, lane 3: HELA whole cell lysate, lane 4: SKOV whole cell lysate, lane 5: HT1080 whole cell lysate, lane 6: COLO320 whole cell lysate, lane 7: HEPG2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLI2 at approximately 118KD. The expected band size for GLI2 is at 118KD.
GLI / GLI1 Antibody - Gli1 antibody Western blot. All lanes: Anti GLI1 at 0.5 ug/ml. WB: Recombinant Human GLI1 Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.
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Polyclonal Rabbit anti‑Human GLI / GLI1 Antibody (aa768‑1106, IHC, WB) LS‑C343972

Polyclonal Rabbit anti‑Human GLI / GLI1 Antibody (aa768‑1106, IHC, WB) LS‑C343972

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C388500
Antibody:
GLI / GLI1 Rabbit anti-Human Polyclonal (aa768-1106) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$431
LS-C343972-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GLI / GLI1 Rabbit anti-Human Polyclonal (aa768-1106) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GLI1 antibody LS-C343972 is an unconjugated rabbit polyclonal antibody to human GLI1 (GLI) (aa768-1106). Validated for IHC and WB.
Target
Human GLI / GLI1
Synonyms
GLI1 | Gli-1 | GLI family zinc finger 1 | Glioma-associated oncogene | Glioma-associated oncogene 1 | Oncogene GLI | GLI | Zinc finger protein GLI1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human GLi1 recombinant protein (Position: Q768-A1106). Human GLi1 shares 79% amino acid (aa) sequence identity with mouse GLi1.
Epitope
aa768-1106
Specificity
Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GLI / GLI1
P08151 NM_005269 NP_005260.1

Publications (0)

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Featured Products

GLI / GLI1 Antibody - Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI / GLI1 Antibody LS‑C180157
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot
GLI / GLI1 Antibody - Anti-GLI1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ GLI / GLI1 Antibody (aa940‑990) LS‑B7244
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Product
GLI / GLI1 Antibody LS‑C150725
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Chromatin Immunoprecipitation
GLI / GLI1 Antibody - IHC of paraffin-embedded Human skin tissue using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
GLI / GLI1 Antibody (aa755‑1106, clone OTI1C1) LS‑C339074
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
GLI / GLI1 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ GLI / GLI1 Antibody (aa755‑1106, clone 4E2) LS‑B13058
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
GLI / GLI1 Antibody - A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI / GLI1 Antibody (N‑Terminus) LS‑C31476
Species: Human
Applications: Western blot
See all GLI / GLI1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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