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Catalog Number Size Price
LS-C744763-100 100 µg (1 mg/ml) $492 
GK / Glycerol Kinase Antibody - Western Blot of Goat anti-Glycerol Kinase Antibody Biotin Conjugated. Lane 1: Glycerol Kinase. Load: 50 ng per lane. Primary antibody: Glycerol Kinase Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Glycerol Kinase.
GK / Glycerol Kinase Antibody - Goat anti Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
GK / Glycerol Kinase Antibody - Western Blot of Goat anti-Glycerol Kinase Antibody Biotin Conjugated. Lane 1: Glycerol Kinase. Load: 50 ng per lane. Primary antibody: Glycerol Kinase Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Glycerol Kinase.
GK / Glycerol Kinase Antibody - Goat anti Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
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Polyclonal Goat anti‑Cellulomonas GK / Glycerol Kinase Antibody (Lyophilized, Biotin, WB) LS‑C744763

Polyclonal Goat anti‑Cellulomonas GK / Glycerol Kinase Antibody (Lyophilized, Biotin, WB) LS‑C744763

Antibody:
GK / Glycerol Kinase Goat anti-Cellulomonas Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Cellulomonas
Format:
Biotin, Lyophilized
Price
Catalog Number
$492
LS-C744763-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
GK / Glycerol Kinase Goat anti-Cellulomonas Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Cellulomonas
Format:
Biotin, Lyophilized

Specifications

Description
Glycerol Kinase antibody LS-C744763 is a biotin-conjugated goat polyclonal antibody to cellulomonas Glycerol Kinase (GK). Validated for ELISA and WB.
Target
Cellulomonas GK / Glycerol Kinase
Synonyms
GK | GKD | GK1 | Glycerokinase | Glycerol kinase
Host
Goat
Reactivity
Cellulomonas (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Glycerol Kinase [Cellulomonas species].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species]. Cross reactivity against Glycerol Kinase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:2500)
  • ELISA (1:1000 - 1:5000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GK / Glycerol Kinase
P32189 NM_000167 NP_000158.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021