Connexin 26 antibody LS-C14525 is an unconjugated rabbit polyclonal antibody to rat Connexin 26 (GJB2 / CX26). Validated for ELISA, IF, IHC and WB.
Rat GJB2 / CX26 / Connexin 26
(tested or 100% immunogen sequence identity)
- IHC - Frozen
- Western blot
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Peptide corresponding to a portion of the cytoplasmic loop of rat connexin 26.
Reacts predominantly with Connexin 26. By immunoblot analysis, a 26kD protein from mouse liver membrane, mouse brain extract and rat brain extract was detected (note: samples were not boiled prior to running SDS-PAGE). A few cross-reactive bands were detected in the 45-50kD range. The identity of these proteins has yet to be confirmed; however, it is possible these bands represent dimeric forms of the Connexin 26 protein. Immunohistochemical staining of frozen sections of human cochlea showed positive reactivity for Connexin 26.
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. ELISA: 0.1-1.0 mg/ml. Western Blot: 0.5 mg/ml. Immunohistochemistry (frozen): 10 mg/ml. Immunofluorescence.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
About GJB2 / CX26 / Connexin 26