Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Immunohistochemistry (Frozen): 3 ug/ml. Western Blot: 1-3 ug/ml. Identifies a single band at ~46kD. Immunofluorescence: 3 ug/ml. Note: For IHC, mice were perfused transcardially with 3 ml pre-fixative (50 mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin), followed by 40mL fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10 ml sucrose wash (10% sucrose in 25 mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10% sucrose, 25 mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.
About GJA3 / CX46 / Connexin 46